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  • br The e ects of HELT fraction emulsions on immune

    2022-05-23


    3.5. The effects of HELT fraction emulsions on immune function
    To assess the effects of F18 emulsions at different concentrations on immune function, we analyzed the SI value, NK cells and B cells. HD-E and MD-E promoted the proliferation of spleen cells as shown in Fig. 7. Moreover, the proportion of NK cells among peripheral blood was sig-nificantly higher in HD-E and MD-E group than others (P < 0.01,
    Fig. 5. Number of lymph node metastases per mouse of each group. Closed circles indicate one mouse with histologically-positive lymphatic metastasis. Opened circles indicate one mouse with histologically-negative lymphatic me-tastasis. The lymph node metastasis rate was significantly reduced in HD-E group (P = 0.006) and MD-E group (P = 0.012). NS: normal saline; CI: Cinobufacini Injection; CE: control emulsion; LD-E: low-dose emulsion; MD-E: middle-dose emulsion; HD-E: high-dose emulsion.
    Table 2 Regional lymph node metastases in different groups.
    17.65 Fig. 7. The spleen index (SI) of each group. The spleen index was significantly
    9.80 saline; CI: Cinobufacini Injection; CE: control emulsion; LD-E: low-dose emul-
    8.33 sion; MD-E: middle-dose emulsion; HD-E: high-dose emulsion.
    Ln.: lymph node; m/d: metastatic/detected; NS: normal saline; CI: Cinobufacini
    Injection; CE: control emulsion; LD-E: low-dose emulsion; MD-E: middle-dose
    emulsion; HD-E: high-dose emulsion.
    Fig. 8A). The proportion of NK cells among spleen cells was also sig-nificantly higher in comparison of LD-E, CE, and NS group (P < 0.05, Fig. 8B). The proportion of Relebactam among peripheral blood and spleen cells was accordingly reduced.
    To evaluate the secrete function of NK cells and B cells, the content
    of IFN-γ and IgG1 in serum and the splenic cell supernatant were also respectively detected (Table 3). The results suggested that HD-E and MD-E significantly enhanced the secretion of IFN-γ in serum and the splenic cell supernatant as well as IgG1 in serum.
    3.6. The potential toxicity of lymphatic chemotherapy with HELT fraction emulsions
    We performed the hematological examination and evaluation of liver and renal function. As shown in Fig. 9, HD-E and MD-E
    Fig. 6. Regional lymph nodes in mouse. A. Enlarged subiliac lymph nodes (red dotted circles) 5 days after tumor challenging. B. Enlarged medial iliac lymph nodes (yellow dotted circle) 21 days after tumor challenging and iliac vessels (yellow arrows). C. Renal lymph nodes (yellow dotted circles) 21 days after tumor challenging. D and E. H&E staining of metastasized lymph node sections showed numerous lymphocytes accumulating in lymph node. D. ×100 magnification. E. ×400 magnification. (For inter-pretation of the references to color in this figure le-gend, the reader is referred to the web version of this article.)
    Fig. 8. The flow cytogram of NK cells among peripheral blood and spleen of each group. A. The proportion of NK cells among peripheral blood was significantly higher in HD-E and MD-E group than others (P < 0.01). B. The proportion of NK cells among spleen cells was also significantly higher in comparison of LD-E, CE, and NS group (P < 0.05). NS: normal saline; CI: Cinobufacini Injection; CE: control emulsion; LD-E: low-dose emulsion; MD-E: middle-dose emulsion; HD-E: high-dose emulsion.
    significantly increased red blood cells, hemoglobin, white blood cells and platelet by activating hematopoietic function. In addition, HD-E and MD-E significantly increased the ratio of lymphocyte, meanwhile, reduced the ratio of neutrophil (Fig. 10A). However, the obvious side effects on liver function were also observed. The level of ALT and AST were significantly higher in HD-E group than other groups (Fig. 10B).