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    Contents lists available at ScienceDirect
    Claudin 7 as a possible novel molecular target for the treatment of pancreatic cancer
    Norimitsu Okui a, Yuko Kamata b, Yukiko Sagawa b, Akiko Kuhara b, Kazumi Hayashi c, Tadashi Uwagawa a, Sadamu Homma b, *, Katsuhiko Yanaga a a Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan
    b Division of Oncology, Research Center for Medical Sciences, The Jikei University School of Medicine, Tokyo, Japan
    c Division of Clinical Oncology and Hematology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    Article history:
    Received in revised form
    Available online 2 November 2018
    Cell proliferation
    Claudin 7
    Gene expression
    Pancreatic cancer
    Cell cycle
    Background/objectives: Pancreatic cancer consists of various subpopulations of cells, some of which have aggressive proliferative properties. The molecules responsible for the aggressive proliferation of pancreatic cancer may become molecular targets for the therapies against pancreatic cancer. Methods: From a human pancreatic cancer cell line, MIA PaCa-2, MIA PaCa-2-A cells with an epithelial morphology and MIA PaCa-2-R cells with a non-epithelial morphology were clonogenically isolated by the limiting dilution method. Gene expression of these subpopulations was analyzed by DNA microarray. Gene knockdown was performed using siRNA.
    Results: Although the MIA PaCa-2-A and MIA PaCa-2-R cells displayed the same DNA short tandem repeat (STR) pattern identical to that of the parental MIA PaCa-2 cells, the MIA PaCa-2-A cells were more proliferative than the MIA PaCa-2-R cells both in culture and in tumor xenografts generated in immu-nodeficient mice. Furthermore, the MIA PaCa-2-A cells were more resistant to gemcitabine than the MIA PaCa-2-R cells. DNA microarray analysis revealed a high expression of claudin (CLDN) 7 in the MIA PaCa-2-A cells, as opposed to a low expression in the MIA PaCa-2-R cells. The knockdown of CLDN7 in the MIA PaCa-2-A cells induced a marked inhibition of proliferation. The MIA PaCa-2-A cells in which CLDN7 was knocked down exhibited a decreased expression of phosphorylated extracellular signal-regulated kinase (p-Erk)1/2 and G1 cell cycle arrest.